ngµç×ÓÓÎÏ·ÐÂÎÅÍøÑ¶£¨Í¨Ñ¶Ô±ÕÅÈñ£©¿ËÈÕ£¬£¬£¬ngµç×ÓÓÎÏ·ÉúÃü¿ÆÑ§Ñ§ÔºÕÅÈñ½ÌÊÚ¿ÎÌâ×éÔÚ¡¶Molecular Cell¡·ÉϽÒÏþÁËÌâΪ¡°Type III CRISPR-mediated flexible RNA excision with engineered guide RNAs¡±µÄÑо¿ÂÛÎÄ£¬£¬£¬±¨µÀÁËÒ»ÏîÖ÷ÒªµÄÊÖÒÕÍ»ÆÆ¡ª¡ªÒ»ÖÖÃûΪSCISSOR£¨Selective Cleavage and Intramolecular Stitching of RNA£©µÄRNA±à¼¹¤¾ß£¬£¬£¬Ê¹ÎÒÃÇÊ×´ÎÄܹ»ÔÚRNA²ãÃæ¾ÙÐÐÎÞаµÄ²Ã¼ôºÍ¸Ä±ä¶ÁÂë¿ò¡£ÕâÒ»ÊÖÒÕÍ»ÆÆ²»µ«Ìî²¹ÁËÏÖÓÐRNA±à¼ÁìÓòµÄ¿Õȱ£¬£¬£¬¸üΪÖÖÖÖÒÆÂëÍ»±äµ¼ÖµÄÒÅ´«¼²²¡ºÍÖ×ÁöÃâÒßÖÎÁÆÌṩÁËȫеĽâ¾ö·½°¸¡£¾ÝϤ£¬£¬£¬¸ÃÑо¿Ð§¹ûÒ²±»Ñ¡ÎªMolecular Cell 3ÔÂ6ÈÕ³öÊéµ±ÆÚµÄ·âÃæÂÛÎÄÖØµãÍÆ¼ö¡£
SCISSOR¹¤¾ßµÄ½¹µãÍ»ÆÆÔÚÓÚÆäÆæÒìµÄ¡°Í¹Æð»·¡±Éè¼Æ£¬£¬£¬ÕâÒ»Á¢ÒìÐÔÉè¼Æ¸³ÓèÁËIIIÐÍ CRISPR-Csm¸´ºÏÎïÔÚRNA±à¼Àú³ÌÖÐǰËùδÓеÄÎÞаÐԺͶàÑùÐÔ¡£¹Å°åÉÏ£¬£¬£¬CRISPR-Csmϵͳ½éµ¼µÄRNA±à¼Öб£´æºÜ´óµÄ¾ÖÏÞÐÔ£¬£¬£¬Ö»ÄÜÒÔ 6ntµÄ±¶Êý¾ÙÐÐRNAƬ¶ÏµÄɾ³ý¡£SCISSORͨ¹ýÒýÈ롰͹Æð»·¡±Éè¼Æ£¬£¬£¬Èà Csm ÏµÍ³Í»ÆÆ6nt±¶ÊýÏÞÖÆ£¬£¬£¬¿É¶ÔRNA¾ÙÐÐí§Òⳤ¶ÈСƬ¶Ñþ³Øý£¬£¬£¬²¢¾«×¼µ÷½â¿ª·ÅÔĶÁ¿ò£¨ORF£©£¬£¬£¬ÊµÏÖÒÆÂëÍ»±äµÄÐÞ¸´¡£
ΪÁËÆÀ¹ÀSCISSORÔÚÐÞ¸´ÒÆÂëÍ»±äÖеÄÖÎÁÆÇ±Á¦£¬£¬£¬ÕÅÈñʵÑéÊÒ°ÐÏòÁËHEXA»ùÒòÖеÄÒ»¸öÁÙ´²Ïà¹ØµÄÒÆÂëÍ»±ä¡£¸ÃÍ»±äÊÇHEXA»ùÒòÍâÏÔ×ÓÉÏÒ»¸ö4¸ö¼î»ù£¨TATC£¬£¬£¬1278ins4£©µÄ²åÈëÍ»±ä£¬£¬£¬ÊÇÒýÆðÌ©-Èø¿Ë˹²¡£¨TSD£©×î³£¼ûµÄÍ»±ä£¬£¬£¬Ô¼Õ¼ËùÓÐTSD²¡ÀýµÄ80%¡£Ñо¿ÖУ¬£¬£¬ÍŶÓʹÓÃSCISSORÊÖÒÕÀÖ³ÉÐÞ¸´ÁËÕâÒ»ÒÆÂëÍ»±ä£¬£¬£¬±ê¼Ç×ÅÀúÊ·ÉÏÊ×´Îͨ¹ýRNAÇиîµÄ·½Ê½Ö±½ÓÐÞÕýÂѰױàÂë»ùÒòÖеÄÖ²¡ÒÆÂëÍ»±ä¡£
SCISSORµÄÄÜÁ¦²»µ«ÏÞÓÚ¡°ÐÞ¸´¡±£¬£¬£¬»¹¿Éͨ¹ýÈËΪÒýÈëÒÆÂëÍ»±ä£¬£¬£¬Òò´ËÔÚ°©Ö¢ÃâÒßÖÎÁÆÖеÄÓ¦ÓÃÒ²ÏÔʾ³öÆäÆæÒìµÄDZÁ¦¡£Í¨¹ýSCISSORƽ̨£¬£¬£¬Ñо¿Ö°Ô±Äܹ»°ÐÏòÖ×Áö¸ß±í´ï»òÕßÌØÒì±í´ïµÄ´Ù°©»ùÒòÒýÈëÒÆÂëÍ»±ä£¬£¬£¬ÌìÉú¾ßÓи߶ÈÃâÒßÔÐԵĶàëÄ¡£Òò´ËSCISSOR¿ÉÒÔ¸úmRNAÒßÃçÁªÓ㬣¬£¬´´Á¢È«ÐµÄͨÓÃÐÍÖ×ÁöÃâÒßÒßÃçÖÎÁÆÕ½ÂÔ£¬£¬£¬¾ßÓÐDZÔ򵀮ͱéÓ¦ÓÃÔ¶¾°¡£

×ܵÄÀ´Ëµ£¬£¬£¬SCISSORÊÖÒÕ²»µ«Ìî²¹ÁËÏÖÓÐRNA±à¼ÁìÓòµÄ¿Õȱ£¬£¬£¬Ò²ÎªÖÖÖÖÒÆÂëÍ»±äµ¼ÖµÄÒÅ´«¼²²¡ºÍÖ×ÁöÃâÒßÖÎÁÆÌṩÁËȫеĽâ¾ö·½°¸¡£ËäÈ»ÃæÁÙһЩÊÖÒÕÌôÕ½£¬£¬£¬µ«Ëæ×ÅÑо¿µÄ³ÖÐøÍÆ½ø£¬£¬£¬SCISSORÓÐÍû³ÉΪRNA±à¼ÁìÓòµÄ½¹µãÊÖÒÕÖ®Ò»£¬£¬£¬Îª»ùÒòÖÎÁÆ¡¢Ö×ÁöÃâÒßÖÎÁƵÈÁìÓò´øÀ´Ç°ËùδÓеÄÍ»ÆÆ¡£
×÷ΪһÏî¾ßÓÐ×ÔÁ¦ÖªÊ¶²úȨµÄÇ㸲ÐÔRNA±à¼ÊÖÒÕ£¬£¬£¬SCISSORÒѾ½á¹¹ÁËÖйú·¢Ã÷רÀûºÍPCT¹ú¼ÊרÀû£¬£¬£¬¾ßÓÐÁÉÀ«µÄÁÙ´²×ª»¯Ô¶¾°¡£
ÕÅÈñÍŶӲ©Ê¿Ñо¿ÉúËïÔ¶·«¡¢ÎâÓ¢Òü£¬£¬£¬Ë¶Ê¿Ñо¿ÉúºÎè÷»ª¡¢Íõ DÓ¨¼°ºîÎĺÀΪ¸ÃÂÛÎĵIJ¢ÁеÚÒ»×÷Õߣ¬£¬£¬ÕÅÈñΪͨѶ×÷Õߣ¬£¬£¬ÕÅÈñÍŶӲ©Ê¿Ñо¿Éú²ÜÓ¡¢ÖÜç÷ð©Ò²¶Ô±¾ÊÂÇé×ö³öТ˳¡£¸ÃÑо¿»ñµÃÁ˹ú¼ÒÖØµãÑз¢×¨Ïî¡¢¹ãÖÝÊлù´¡ÓëÓ¦Óûù´¡Ñо¿×¨Ìâ¿Æ¼¼Ý¼Ó¢¡°Á캽¡±ÏîÄ¿ºÍ¹ãÖÝÊпÆÑ§ÊÖÒÕ¾ÖÖØµãÑз¢ÍýÏëµÄ×ÊÖú¡£
ÂÛÎÄÁ´½Ó£º£º£º
https://www.cell.com/molecular-cell/fulltext/S1097-2765(25)00055-3