ngµç×ÓÓÎÏ·ÐÂÎÅÍøÑ¶£¨Í¨Ñ¶Ô±Ñ»ª£©TP53 (p53)ÊÇ×îÖ÷ÒªµÄÖ×ÁöÒÖÖÆÒò×Ó£¬¹ØÓÚά³Öϸ°û»ùÒò×éÍêÕûÐÔÖÁ¹ØÖ÷Òª¡£¡£ÎªÓ¦¶Ô DNA ËðÉË£¬p53 ÂѰױ»¼¤»î²¢×÷Ϊת¼Òò×Óµ÷¿Ø´ó×ÚµÄÂѰ×ÖʱàÂë»ùÒò¡¢¡¢¡¢Î¢RNA£¨miRNA£©ºÍ³¤·Ç±àÂëRNA£¨lncRNA£©µÈµÄ±í´ï¡£¡£ÎÒÃÇǰÆÚÑо¿·¢Ã÷ÁËp53µ÷¿ØµÄLTRÄæ×ª×ù×ÓÑÜÉúµÄlncRNA£¨LTR-lncRNA£©Ôö½øDNAÍ¬Ô´ÖØ×éÐÞ¸´µÄй¦Ð§£¨EMBO Reports, 2019£©¡£¡£ÓÐȤµÄÊÇ£¬×î½üµÄÑо¿·¢Ã÷һЩlncRNA¿ÉÒÔ½øÒ»²½±àÂë¶àëÄ£¨Î¢ëÄ£©ÔÚÖ÷ÒªµÄÐÄÀíÓ벡ÀíÀú³ÌÖÐʩչµ÷¿Ø¹¦Ð§¡£¡£È»¶ø£¬p53µ÷¿ØµÄlncRNAÊÇ·ñ¿ÉÒÔ±àÂë¶àëļ°×÷Ϊ¶àëÄʩչÖ÷ÒªµÄÉúÎïѧ¹¦Ð§ÈÔÓдýÆÊÎö¡£¡£
¿ËÈÕ£¬ngµç×ÓÓÎÏ·ÉúÃü¿ÆÑ§Ñ§ÔºÑ»ª½ÌÊÚ/ÇüÁ¼ðÀ½ÌÊÚ¿ÎÌâ×éʹÓöà×éѧÊÖÒÕ·¢Ã÷Ò»Åú³¤Á´·Ç±àÂëRNA£¨lncRNA£©±àÂëµÄ¶àëÄÊÇp53ÐźÅͨ·ÖеÄгÉÔ±£¬²¢Õ¹ÏÖÕâЩ¶àëÄÔÚp53ÐźÅͨ·µÄ¹¦Ð§Óëµ÷¿ØÍøÂç¡£¡£ÍŶÓͨ¹ý¹¹½¨DNAËðÉËÐÞ¸´Ï¸°ûÄ£×Ó²¢Á¬ÏµºËÌÇÌåͼÆ×²âÐòÊÖÒÕ£¨Ribo-seq£©¡¢¡¢¡¢RNA-seq¡¢¡¢¡¢CRISPR/Cas9ÊÖÒÕ¼°RNA×éѧÆÊÎöÊÖÒÕ£¨Í¼1£©£¬ÔÚÈËÀàϸ°ûÖÐϵͳµØÅжϵ½ÁË300¶à¸öÊÜp53µ÷¿ØµÄlncRNAºÍ200¶à¸ö¾ßÓбàÂëDZÄܵÄlncRNA¡£¡£

ͼ1£º£º£ºÁªºÏ¶à×éѧÊÖÒÕÅжÏp53µ÷¿ØÇÒ¾ßÓбàÂëDZÄܵÄlncRNA
¸ÃÑо¿Ëæ»úÌôѡһÅúlncRNA¾ÙÐÐʵÑéÑéÖ¤£¬Ö¤ÊµÁË15¸öÊÜp53µ÷¿ØµÄlncRNA±àÂëµÄ¶àëÄ¡£¡£±ðµÄ£¬Í¨¹ýʹÓöàÖÖÖÊÆ×ÊÖÒÕ£¨shotgun MS¡¢¡¢¡¢PRM MSºÍMRM MS£©ºÍ¿¹ÌåÖÆ±¸µÈÊÖÒÕ·¢Ã÷¶à¸ö¶àëÄÔÚÈËÀàϸ°ûÊÇÄÚÔ´ÐÔ±í´ïµÄ¡£¡£½øÒ»²½µÄ¹¦Ð§È±Ê§¼°»ñµÃʵÑé·¢Ã÷ÊÜp53µ÷¿ØµÄTP53LC02ºÍTP53LC04¶àëÄÄܹ»ÒÖÖÆ¶àÖÖ°©Ï¸°ûµÄÔöÖ³£¬ÇÒʩչ×÷ÓõÄÊǶàëÄ·Ö×Ó¶ø·ÇlncRNA·Ö×Ó¡£¡£¸ÃÑо¿»¹Í¨¹ýʵÑé֤ʵTP53LC04¶àëÄ¿ÉÒÔͨ¹ýµ÷¿ØÏ¸°ûÖÜÆÚÈ¥¼ÓÈëp53½éµ¼µÄÖ×ÁöÒÖÖÆ¡£¡£¸ÃÑо¿Õ¹ÏÖÁËlncRNA±àÂëµÄ¶àëÄÔÚp53µ÷¿ØÍøÂçÖг䵱µÄȫнÇÉ«£¨Í¼2£©£¬ÍØÕ¹ÁËÈËÃǶÔp53µ÷¿ØÍøÂçµÄÊìϤ£¬²¢½«Îª°©Ö¢ÓÐÓÃÖÎÁÆÌṩÁËеÄDZÔڰе㡣¡£

ͼ2£º£º£ºp53µ÷¿ØµÄlncRNA±àÂë¶àëÄÔÚ°©Ï¸°ûÔöÖ³ÖеĹ¦Ð§
¸ÃÑо¿ÒÔ¡°TP53-inducible putative long noncoding RNAs encode functional polypeptides that suppress cell proliferation¡±ÎªÌâ½ÒÏþÔÚ¹ú¼ÊѧÊõÆÚ¿¯Genome ResearchÉÏ¡£¡£ngµç×ÓÓÎÏ·ÉúÃü¿ÆÑ§Ñ§ÔºÑ»ª½ÌÊÚ¡¢¡¢¡¢ÇüÁ¼ðÀ½ÌÊÚ¡¢¡¢¡¢Àî±óÌØÆ¸¸±Ñо¿Ô±¼°ôßÄÏ´óѧºÎÇì褽ÌÊÚΪ±¾ÎÄÅäºÏͨѶ×÷Õߣ¬ÐíÎÄÀö²©Ê¿¡¢¡¢¡¢Áõ³©²©Ê¿Éú¡¢¡¢¡¢µË±ø²©Ê¿ºÍÁÖÅô»Ô˶ʿΪ±¾ÎIJ¢ÁеÚÒ»×÷Õß¡£¡£¸ÃÑо¿»ñµÃ¹ú¼ÒÖØµãÑз¢ÍýÏë¡¢¡¢¡¢¹ú¼Ò×ÔÈ»¿ÆÑ§»ù½ðµÈÏîÄ¿×ÊÖú¡£¡£
ÂÛÎÄÁ´½Ó£º£º£ºhttps://genome.cshlp.org/content/32/6/1026